ARF1 antibody, rabbit polyclonal

Background

The ARF1 (ADP-ribosylation factor 1) protein is localized to the Golgi apparatus and has a central role in

intra-Golgi transport. It is a small GTPase that undergoes a GDP/GTP nucleotide exchange cycle and it is an

important regulator of cellular trafficking. Gene encoding ADP-ribosylation factor and similar to other ARFs and

ARF-like proteins. A member of ARF GTPase family. Arabidopsis has 21 known members, known to be essential

for vesicle coating and uncoating and functions in GTP-binding. The gene is shown to play a role in cell division,

cell expansion and cellulose production using antisense construct.

Other names: ADP-RIBOSYLATION FACTOR, ADP-RIBOSYLATION FACTOR 1, ADP-RIBOSYLATION

FACTOR 1A, ARF1, ATARF, ATARF1, ATARFA1AAT1G23490

Immunogen: Rabbit polyclonal antibodies were produced by immunizing animals with a recombinant GST fusion

of full length of Arabidopsis thaliana ARF1 (AT2G47170). Antibodies were purified by affinity purification using

immunogen.

Concentration: 1.0mg/ml

Tested applications: Western blot (WB), IF

Expected/apparent MW: 21 kDa

Species specificity: Arabidopsis thaliana

Recommended dilution: 1 : 500-2000 (WB, detect endogenous protein ), 1: 100 - 1: 500 (IF)(optimal dilutions

should be determined by the end use.)

Storage Buffer: PBS Buffer, pH7.4

Storage: Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Stable for 2

years from date of receipt. Aliquot to avoid repeated freezing and thawing.

* Please note: FOR RESEARCH USE ONLY